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six%) have been categorised as helpful, in which virtually just about every mutation was most popular over the wild-kind residue. as compared, the volume of advantageous positions lessened to ten in +Lon selection, with only 3 surface-uncovered positions (E48, T68, D127) popular concerning The 2 useful sets. concurrently, the quantity of Restricted positions elevated from 42 to 67 Together with the reintroduction of Lon into the choice strain (determine 2C). These effects assistance the summary that Lon exercise broadly penalizes mutations, including a large subset with the beneficial mutations. Overall, the variations on modulating Lon exercise bring about a model by which upregulating Lon improves constraints on DHFR, and the mutational landscape modifications from becoming permissive when Lon is absent to remaining a lot more restricted when Lon is current (Figure 2D).
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